Articles
RSSEconomic Benefits Of Protein A Alternatives
June 1, 2007
Click Here To Download:•Article: Economic Benefits Of Protein A Alternatives
Affinity chromatography (AC) on protein A–based sorbents has been used for more than a generation by drug manufacturers to purify protein therapeutics. AC is based on the ability of a protein or other biopolymer to bind with a natural or synthetic ligand. When used in conjunction with other chromatography steps, it provides an effective way of isolating desired antibodies for downstream drug production.
It's no secret, however, that protein A–based sorbents are expensive, a fact that puts pressure on manufacturing to use such sorbents as efficiently as possible to keep overall production costs under control. The expense is compounded by other factors that limit the usefulness of protein A–based sorbents. They have low cleaning tolerances compared with chemical-ligand (e.g., ion-exchange) sorbents, and the leaching of protein A fragments during antibody purification remains a significant problem despite attempts to improve engineering.
Reprinted with permission from BioProcess International 3(5):68-70 (May 2005) Click Here To Download:•Article: Economic Benefits Of Protein A Alternatives
SOURCE: Pall Life Sciences
