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RSSImprove Product Recovery During Cell Harvesting
April 30, 2007
Click Here To Download:•Article: Improve Product Recovery During Cell Harvesting
Development efforts for vaccines, therapeutic proteins, monoclonal antibodies, and gene therapies all rely heavily on cell harvesting. Given the growing global demand for new therapies — particularly blockbuster drugs — manufacturers face severe production capacity issues. The need to increase the efficiency of product recovery during upstream processing is critical. Current global fermentation capacity cannot come close to meeting the projected demand, and large new biomanufacturing plants take a long time to come on line.
In the past, manufacturers might have been satisfied with a 50–60% product recovery rate, but such low figures are no longer acceptable. Not only must recovery be maximized to obtain the highest possible volumetric throughput, but separation techniques must also ensure that product integrity is not compromised. Product consistency, both within batches and from batch-to-batch, is another key goal because it has serious implications for validation.
Several new membrane technologies have been developed to improve the efficiency of tangential flow filtration (TFF) operations and have doubled product recoveries in beta testing (as described in the "Results" box). One such technology is a compact TFF cassette device that uses polyethersulphone membranes and screens to prevent product channeling and improve flow properties. The other technology is an enhancement to hollow-fiber modules for cell perfusion operations, using a new type of nonbinding PVDF (polyvinylidene difluoride) membrane coupled with backpulse capabilities.
Reprinted with permission from BioProcess International V1 - No.4 pp 62-65 (April 2003) Click Here To Download:•Article: Improve Product Recovery During Cell Harvesting
SOURCE: Pall Life Sciences
