Application Note
Microdialysate Microbore Analysis Of Catecholamine And Metabolites
Analysis of catecholamines and their metabolites in small volume samples such as those obtained from microdialysis.
The analysis of catecholamines and their metabolites from brain microdialysates poses three technical problems: the small sample size generated by this sampling technique, possible low levels of target analytes, and the need to elute an array of compounds in a reasonable period of time. Microbore columns can solve all these problems. Sample volumes for microbore columns are typically in the 1-10 µL range, which is less than the sample size usually collected during microdialysis. Microbore columns also provide rapid and efficient separations, allowing the determination of catecholamines and their metabolites in one isocratic run.
Here we present several catecholamine separations on a BASi 150 x 1 mm UniJet SepStik microbore HPLC column. To demonstrate the versatility of this column, we use three mobile phases, each with slightly different properties. samples such as those obtained from microdialysis.
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