Product/Service
Protein A Ceramic HyperD F Affinity Chromatography Sorbent
For Large-scale Antibody Purification
- Ideal for large-scale antibody purification
- High binding capacity for human and murine IgGs
- High selectivity with no non-specific binding
- Rigid
- Low backpressure
- Easy to pack
BioSepra Protein A Ceramic HyperD® F sorbent available from Pall is a high capacity affinity sorbent designed for process-scale purification of immunoglobulins G. The sorbent combines ease of use with high binding capacity and excellent scalability. Antibodies of greater than 95% purity are isolated from cell culture supernatant or ascites fluid in a single chromatographic step. Dilute feedstock (~100 µg IgG/mL) may be applied without preliminary concentration. BioSepra Protein A Ceramic HyperD F sorbent is prepared using a rigid proprietary ceramic bead. Recombinant Protein A is immobilized to a specially formulated hydrogel within the porous ceramic bead.
The ceramic bead, the hydrogel, and the linkage used for the Protein A coupling are chemically stable over a broad range of conditions. Cleaning-in-place and sanitization procedures using sodium hydroxide may be employed with only limited loss of binding capacity. The unique, multi-point coupling chemistry provides a sorbent that exhibits very low leakage of recombinant Protein A.
The Protein A ligand is of recombinant origin and is produced in strict compliance with cGMP requirements. The recombinant Protein A is purified by standard chromatographic processes and is never in contact with immunoglobulins.
- Ask a question.
- Request more detailed information or literature.
- Discuss your current project/application.
- Request a quote.
- Locate a distributor in your area.
- Schedule a demo.
