Application Note
Tighter pH Control In Chromatography And Purification Systems
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•Application Note: Tighter pH Control In Chromatography And Purification Systems
By Emerson Process Management, Rosemount Analytical Liquid Division
Biopharmaceutical Chromatography Systems are designed for separating and purifying proteins and bio-engineered products. Flow systems are compact in design to maximize throughput. No dead legs in process pipe can exist since unswept areas are more challenging to completely clean, as well as delaying product throughput by decreasing flow rates. The systems must maintain a hygienic design. Wetted surface finish must be < 20 µinch Ra and material traceability is important to maintain system integrity.
pH measurement plays an important role in the purification process, providing feedback control of buffer and effluent feed through the column. The system's wetted components must withstand Cleaned in Place (CIP) and Steamed in Place (SIP) cycles, which can stress a sensor with high heat and caustic. The pH sensor must be able to make accurate and stable pH measurements after the cleaning cycles.
Click Here To Download:•Application Note: Tighter pH Control In Chromatography And Purification Systems
