Allegro™ Single-Use Tangential Flow Filtration System

Pall’s new single-use TFF system is the latest addition to the Allegro range of integrated single-use bioprocessing solutions. It combines the advantages of Allegro single-use manifolds with an engineered automated system design, providing reliable control of key parameters and ease-of-use in manufacture.

The Allegro single-use TFF system is designed specifically for the incorporation of single-use components. It is not a disposable version of a traditional TFF system design because new concepts for ease-of-use in single-use processing have been used to achieve this unique design. This includes careful consideration of the layout and installation, allowing full access to components from one side of the system.

Attributes

•     Good engineering design provides reliability, control and ease-of-use
•     Full automation provides control and monitoring of key process parameters
•     Gamma-irradiated manifolds with pre-calibrated sensors ensure quick installation

System Concept

Single use technology is widely accepted in bioprocessing, with key benefits being the elimination of cross contamination and cleaning, and greater system flexibility.

Tangential Flow Filtration (TFF) is more complex than many other unit operations for which single-use approaches have been adopted. In TFF, cleaning of the TFF casettes and system is essential to maintain flux and minimize the risk of cross contamination in a multi-product facility.

The Allegro system provides a platform onto which five single-use manifolds are attached. The five manifolds (feed, retentate, permeate, retentate biocontainer and recovery) are provided gamma irradiated and ready to install on the system.

The single-use flowpaths have incorporated single-use sensors where possible. These sensors are manufactured to defined tolerances and minimize downtime in system set up.