Product/Service

BioPAT® Fundalux®

Source: Sartorius Stedim Biotech

Fundalux

BioPAT® Fundalux – For online measurement of turbidity and total biomass in bacterial fermentation and mammalian cell cultures.  Designed for use in R&D, as well as in pilot- and production-scale..  Can be used for online triggering of control loops for advanced automated fermentation.

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BioPAT® Fundalux® is an absorption-based photometric probe that uses light from 840 nm to 910 nm (NIR) to measure the turbidity in bioreactors and fermenters. A precisely defined and constant LED light beam passes through the process medium from either side of the gap on the probe’s shaft. Its hermetically sealed photodiode detects attenuation of light intensity, which is caused when light is absorbed by cellular mass, dissolved compounds and insoluble material. The 180° constant direct beam optical path lengths (OPL) allow maximum utilization of LED light intensity.

The changes in detected light intensity are caused by different degrees of absorption by materials in the medium, which is described by the Beer-Lambert law. This states that the logarithm of the transmission loss is proportional to the concentration of a substance. In addition, as BioPAT® Fundalux® operates according to the transmittance principle in the NIR range, it is not affected by color changes in the cultivation medium. As a result, the probe measures the concentration of total biomass accurately and constantly throughout a process based on the respective medium properties.

Benefits:

  • Designed for use in R&D, as well as in pilot- and production-scale
  • Monitoring cell growth in bacterial fermentation and mammalian cell cultures
  • Online triggering of control loops for advanced automated fermentation

 

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