Comparative Evaluation Of Automated Flash Chromatography And Preparative HPLC For Bench-Scale Purification Of A Broad Range Of Sample Types
By Bopanna N.K., Chitra Sundararajan, and Mark Jacyno
The goal of a purification chemist is to deliver suitable quantities of high purity compounds in the shortest possible time. Two of the most popular purification techniques are Automated Flash Column Chromatography (AFCC) and Preparative HPLC. Traditionally, AFCC is characterized by the ability to load large amounts of material and has short purification times, while Prep HPLC is valued for high resolution separations resulting in very pure products. As a result, AFCC is often used as a preliminary technique whereby the crude sample is pre-purified before final high resolution purification using Prep HPLC.
With the recent advances in AFCC instruments and cartridges, the gap between ‘high speed’ flash purification and ‘high efficiency’ Preparative HPLC purification is diminishing. In many cases AFCC can deliver large quantities of product with comparable purity to Prep HPLC, in significantly less time and at lower cost.
In this study, we evaluate the productivity advantages of AFCC over Prep HPLC, in terms of time, solvent and overall cost savings. We also demonstrate the benefits of AFCC both as a complementary technique to Prep HPLC, as well as a highly versatile stand-alone technique to deliver high purity separations in a cost-effective manner.