Products and Services

RSS

Hydroxyapatite Chromatography Sorbent

• Effective purification mechanism in a variety of processes
• High porosity
• Easy cleaning
• Used in large scale step

Biosepra HA Ultrogel® sorbent available from Pall is a hydroxyapatite agarose composite sorbent for the separation of biomolecules from research and development scale to manufacturing.

Hydroxyapatite chromatography is considered to be a "pseudo-affinity" chromatography, or "mixed-mode" ion exchange. It has proven to be an effective purification mechanism in a variety of processes, providing biomolecule selectivity complementary to more traditional ion exchange or hydrophobic interaction techniques. HA Ultrogel is easy scalable and is currently used in research scale to multi-liter column applications.

HA Ultrogel hydroxyapatite sorbent is composed of cross-linked agarose beads with microcrystals of hydroxyapatite entrapped in the agarose mesh. The particle size ranges between 60 and 180 µm. The agarose moiety in HA Ultrogel is chemically stabilized with epichlorohydrin under strongly alkaline conditions. Thus HA Ultrogel can be regularly treated with 0.1 – 1.0 M NaOH for regeneration and sanitization. This creates glycerol bridges between the polysaccharide chains and gives the sorbent beads an excellent rigidity and stability to pH and ionic strength changes, as well as to high temperature.

HA Ultrogel porosity is comparable to an agarose gel, with an exclusion limit for globular proteins of 5,000,000 daltons. This macroporosity avoids any molecular sieving effect during the separation (see Figure 1).

The sorbent is shipped in 1 M NaCl containing 20% ethanol and is available in a range of package sizes. Special packaging to meet specific manufacturing requirements is available on request.