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MEP HyperCel Ligands
•Datasheet: MEP HyperCel Ligands
Hypercel™ is composed of cellulose matrix to which the ligand is linked. The cellulose bead confers high porosity, chemically stability and low non-specific interaction.
For MEP Hypercel, 4-mercapto-ethyl-pyridine (4-MEP) ligand was chosen for its high selectivity and capacity for antibodies, and for its pKa which is 4.8. It contains a hydrophobic tail and ionizable headgroup. Adsorption is based on mild hydrophobic interaction, and is achieved without addition of lyotropic or other salts. Desorption is based on charge repulsion, it is performed by reducing the pH.
MEP Hypercel is a high capacity, high selectivity sorbent specially designed for the capture and purification of monoclonal and polyclonal antibodies from a broad range of sources, such as animal sera, ascites fluid and cell culture supernatant.
The IgG binding capacity of MEP Hypercel is essentially independent of subclass or species.
MEP Hypercel is also an alternative to conventional Hydrophobic Interaction Chromatography:
- Binds and elutes by a unique mechanism (Hydrophobic Charge Induction)
- Requires less lyotropic salt than conventional HIC (i.e., Phenyl) for protein binding and elution: lower pro¬cess costs and waste disposal benefits
- Robust reproducible manufacturing.
Features and Benefits
- Designed for direct capture of monoclonal and polyclonal IgGs at physiological pH, no dilution
- High binding capacity, regardless of IgG subclass or species
- Alternative to Protein A-based sorbents for antibody production
- Eliminates need to monitor and remove leached Protein-A
- Convenience — Easy to pack and run, can be operated at low backpressure (< 3 bar) in mL or multi-liter packings, used from laboratory to process-scale
- Preserves antibody integrity
- Facilitates development of simplified, robust purification without adjustment of pH or ionic strength of the feedstock
- Chemically stable to base: easy long term cleaning with 1M NaOH gives this material a significant advantage over Protein A-based sorbents
- Cost – Provides significant savings in sorbent cost: 25% the cost of typical protein A and long service life.
Applications
- Direct antibody capture from cell culture supernatant
- Purification of monoclonal IgGs from ascites fluid
- Separation of immunoglobulins from colostrum or sweet whey
- Separation of antibodies expressed in transgenic vegetal or animal organisms
- Polyclonal or monoclonal IgG from various classes and species (polyclonal bovine Ig; mouse IgG2a; hu-IgG1; some Fc-fusion proteins and IgA)
- Alternative to conventional HIC for recombinant protein purification
Table 3: Main Properties of MEP Hypercel and MBI Hypercel
| - | MEP Hypercel |
| Particle size | 80 – 100 µm |
| Dynamic binding capacityfor hu IgG (10% breakthrough) |
20 mg/mL(1) |
| Ligand | 4-Mercapto-ethyl-pyridine |
| Working pH | Adsorption: pH 7.0 – 9.0 Elution: pH 5.8 – 4.0 |
| Cleaning pH | 3 – 14 |
| Pressure resistance | 3 bar (44 psi) |
| Typical working pressure | 1 bar (14 psi) |
(1)Determined using 5 mg /mL human IgG in PBS, 60 cm/h
(2)Capacity varies according to the IgG type, the concentration and the resistance time, i.e., for hu IgG (5 mg/mL) in 50 mM sodium acetate, pH 5.5, capacity is equal to 25 mg/mL for 5 min. residence time.
Ordering Information
| Product | Catalog. No | Size |
|---|---|---|
| MEP Hypercel | 12035-069 | 5 mL |
| - | 12035-010 | 25 mL |
| - | 12035-028 | 100 mL |
| - | 12035-036 | 1 L |
| - | 12035-040 | 5 L |
| - | 12035-044 | 10 L |
•Datasheet: MEP HyperCel Ligands
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