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Hydrophobic Charge Induction Chromatography (HCIC) Sorbent

• Specially designed for purification of antibodies
• Direct sample loading without any adjustment of pH or ionic strength
• High purity achieved in a single step
• High IgG capacity, independent of subclass or species
• Easy cleaning with sodium hydroxide

MEP Hypercel™ is a high capacity, high selectivity sorbent specially designed for the capture and purification of monoclonal and polyclonal antibodies.
MEP Hypercel supports efficient capture and purification of antibodies from a broad range of sources, such as animal sera, ascites fluid and cell culture supernatant. A variety of cell culture formulations are readily accommodated, including protein-free, albumin-supplemented and serum-supplemented media. In contrast to Protein A sorbents, IgG binding capacity on MEP Hypercel is essentially independent of subclass or species. "Weakly-binding" variants (e.g., murine IgG1) are well retained.
MEP Hypercel provides significant benefits at both laboratory and process-scale:

Sample Preparation is Reduced to Clarification

• Feedstock may be applied without adjustment. Binding occurs at neutral pH, and is independent of ionic strength. No need to add lyotropic or other salts.
• Concentration of dilute samples is not necessary. Efficient capture is achieved even with feedstocks as dilute as ~50 – 100 µg IgG/mL.

Rapid and Efficient Sample Processing

• Large volumes of sample can be processed rapidly and efficiently. Dynamic binding capacities = 30 mg IgG per ml of sorbent (at 10% breakthrough) are routinely achieved
• High purity in one step. Product purities of 70-90%, or greater, are typically achieved
• Gentle elution reduces the risk for antibody aggregation and eliminates the need for desalting or diafiltration. A simple lowering of the pH to 4, at low ionic strength, will elute the antibody.