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•Datasheet: Q, S, DEAE, CM Ceramic HyperD F, 20 Sorbents

Improve throughput with Ceramic HyperD enhanced diffusion chromatography sorbents.

Ceramic HyperD ion-exchangers are high capacity sorbents specially designed for the efficient and scalable purification of biomolecules from a variety of feedstocks. Of particular value in process-scale applications, these sorbents are designed to maintain high dynamic binding capacity (DBC) under conditions where conventional sorbents display significant capacity or productivity limitations. They employ a high-capacity hydrogel polymerized within the gigapores of a rigid ceramic bead. Ceramic HyperD sorbents do not shrink or swell with changes in pH or conductivity. Abundant ion-exchange sites in the hydrogel are highly accessible to protein molecules. Proteins diffuse rapidly within the hydrogel, facilitating rapid uptake of product. This mechanism of mass–transfer, known as enhanced diffusion, allows the sorbent to operate free of operational constraints typically encountered with conventional macroporous ion-exchange sorbents.

The Ceramic HyperD 20 particle size is more adapted for process development, polishing steps or rapid separations when a higher resolution is required. Two chemistries, Q and S, are available.

Ceramic HyperD F sorbents are ideally suited for purification of biomolecules in research, scale-up and full-scale pharmaceutical manufacturing. Four chemistries are available: Q, S, DEAE and CM.

Features and Benefits

• Product capture at high linear velocity — Dynamic binding capacity (DBC) with Ceramic HyperD ion-exchangers is virtually independent of linear velocity at values typically used — or sought — in laboratory or process scale separations
• Efficient harvest from dilute feedstock — Higher dynamic binding capacity is obtained with dilute feedstock rather than with concentrated feedstock contrary to that observed with traditional sorbents. Product can be harvested from dilute feedstock without the need for preliminary concentration, thereby enhancing the productivity of the overall purification scheme
• Convenient — With absolute dimensional stability and excellent pressure flow characteristics, easy-to-pack Ceramic HyperD F sorbents form very stable beds. They do not swell or shrink in response to change in pH, ionic strength or flow rate. Indeed, the sorbents are stable at pressure up to 70 bar (1,000 psi)
• Robust — Sorbents can be treated with NaOH (i.e., 5-column volume of 0.5 M NaOH, 1 hour con¬tact time, 25 °C (77 °F) followed by a re-equilibration with sterile pyrogen free water) for efficient cleaning and sanitization
• Go fast without sacrificing capacity — Higher loads, better throughput. Ceramic HyperD ion-exchangers deliver high dynamic binding capacity at high linear velocity. No significant decline in DBC for BSA is observed when increasing the linear velocity from 50 cm/h to more than 650 cm/h and when reducing residence time from 3 minutes to 0.4 minutes. So high flow velocity, short resi¬dence time, reduced bed volume, and reduced buffer volume, support high productivity and enhanced process economics
• Achieve high flow velocities using standard chromatography equipment — The rigid ceramic skeleton of Ceramic HyperD F allows work at high linear flow velocities (typically over 300 cm/h) with low or moderate backpressure (typically less than 3 bar) without compression or shrinkage. Stan¬dard low pressure chromatography pumps and columns can be used, contributing to overall process economy

CM Ceramic HyperD F – IgG Purification

• Dedicated for direct capture of monoclonal antibodies from unmodified feedstock:
• The high ionic capacity permits direct loading of feedstock (no adjustment of ionic strength, no concentration required)
• CM Ceramic HyperD F exhibits high capacity (> 20 mg/mL of monoclonal antibodies) in physiological ionic strength conditions
• > 90% purity in one step from BSA-free culture supernatant
• Provide an alternative to Protein A affinity chromatography (to avoid protein A leakage and to reduce aggregate formation due to milder elution pH)

Applications

• Direct capture of biomolecules from a variety of feedstocks
• Polypeptides, IgG, albumin purification
• Large-scale purifications
• Purification of monoclonal antibodies from ascites or cell culture
• Plasmid purification
• Process polishing steps
• Rapid high resolution purification (20 µm grade)
• Ideal for direct capture of antibodies from clarified, unadjusted feedstock

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•Datasheet: Q, S, DEAE, CM Ceramic HyperD F, 20 Sorbents