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•Datasheet: Sorbent Selection Guide

Mixed Mode Sorbent Chromatography

Sorbent	Ligand	Particle Size (µm)	Working pH	Cleaning pH	Applications
MEP Hypercel	4-Mercapto-ethyl-pyridine	80 – 100	Adsorption: pH 7.0 – 9.0* Elution : pH 4.0–5.8*	3 – 14	• Polyclonal and monoclonal antibodies of most classes, sub-classes and species • Enzymes and recombinant Proteins • Direct capture of polyclonal and mono¬clonal antibodies
MBI Hypercel	2-Mercapto-5-benzimidazole sulfonic acid	80 – 100	Adsorption: pH 5.0 – 5.5* Elution : pH 8.0 – 9.5*	3 – 14	• Polyclonal and monoclonal antibodies of most classes, sub-classes and species • Enzymes and recombinant Proteins • Direct capture of polyclonal and mono¬clonal antibodies

Ion Exchange Chromatography

Process Development Scale — Polishing

Sorbent	Chemical group	Particle Size (µm)	Working pH	Cleaning pH	Applications
Q, S Ceramic HyperD 20	Quaternary amine (Q) Sulfopropyl (S)	20 (av.)	2 – 12	1 – 14	• Polypeptides • Plasmid purification • High resolution for recombinant proteins

Production Scale

Sorbent	Chemical group	Particle Size (µm)	Working pH	Cleaning pH	Applications
CM HyperZ Q HyperZ	Carboxymethyl (CM) Quaternary amine (Q)	75 (av.)	4 – 13	1 – 14	• Expanded bed and packed bed separa¬tions
Q, S, DEAE, CM Ceramic HyperD F	Quaternary amine (Q) Sulfopropyl (S) Diethylaminoethyl (DEAE) Carboxymethyl (CM)	50 (av.)	2 – 12	1 – 14	• Recombinant proteins • Plasmid purification • Proteins, Vaccines • Monoclonal antibodies • Capture step • Immunoglobulin purification
DEAE, CM, SP Trisacryl M, LS	Diethylaminoethyl (DEAE) Carboxymethyl (CM) Sulfopropyl (SP)	M: 40 – 80 LS: 80 – 160	1 – 11	1 – 11	• Lysozyme, peptides • Insulin, immunoglobulins • Plasma fractionation • Rec. proteins
DEAE, SP Trisacryl Plus M, LS	Diethylaminoethyl (DEAE) Sulfopropyl (SP)	M: 40 – 80 LS: 80 – 160	1 – 11	1 – 14	• Immunoglobulins • Glycoproteins
DEAE, SP Spherodex LS	Diethylaminoethyl (DEAE) Sulfate (SP)	100 – 300	1 – 12	-	• High productivity separation • Human serum albumin • Plasma fractionation • Recombinant proteins
QMA Spherosil M, LS	Quaternary amine partially hydrophobic	M: 40 – 100 LS: 100 – 300	1 – 12	-	• Albumin purification from human serum • Proteins

Size Exclusion Chromatography

Sorbent	Particle Size (µm)	Exclusion limit (dt)	Fractionation range (dt)	Working pH	Applications
Trisacryl GF05 M, LS	40-80 (M) 80-160 (LS)	3,000	200 – 2,500	1 – 11	• Separation of small molecules • Desalting
Trisacryl GF2000 M, LS	40-80 (M) 80-160 (LS)	20,000,000	10,000 – 15,000,000	1– 11	• Purification of macromolecules • Affinity chromatography
Ultrogel AcA202	60-140	22,000	1,000 – 15,000	3 – 10	• Desalting
Ultrogel AcA54	-	90,000	5,000 – 70,000	-	-
Ultrogel AcA44	-	200,000	10,000 130,000	-	• Fractionation, purification
Ultrogel AcA34	60-140	750,000	20,000 – 350,0000	3 – 10	• Molecular weight determination
Ultrogel AcA22	-	3,000,000	100,000 – 1,200,000	-	-

Hydroxyapatite Chromatography

Sorbent	Ligand	Particle Size (µm)	Working pH	Cleaning pH	Applications
HA Ultrogel	Hydroxyapatite crystals	60 – 180	5 – 13	5 – 14	• Immunoglobulin separation • Glycoproteins, vaccines

Affinity Chromatography

Sorbent	Ligand	Particle Size (µm)	Working pH	Cleaning pH	Applications
Protein A Ceramic HyperD F	Recombinant Protein A	50 (av.)	2 – 11	2 – 13	• Immunoglobulins • Murine monoclonal antibodies
Blue Trisacryl M	Cibacron blue	40 – 80	1 – 10	1 – 11	• Albumin, Enzymes • Growth hormones • Growth factors • Interferon
Heparin HyperD M	Porcine Heparin	80 (av.)	3 – 13	-	• Coagulation factors • Growth hormones • Enzymes • Lipoproteins
Lysine HyperD	L-lysine	70 (av.)	3 – 13	-	• Plasminogen • Glycoproteins

Solvent-Detergent Chromatography

Sorbent	Ligand	Particle Size (µm)	Working pH	Cleaning pH	Applications
SDR HyperD	Hydrophobic polymer moiety	40 – 100	2 – 12	-	• Solvent detergent removal from biological liquids after virus inactivation

Hydrophobic Interaction Chromatography

Sorbent	Ligand	Particle Size (µm)	Working pH	Cleaning pH	Applications
Methyl Ceramic HyperD F	Methyl	50 (av.)	2 – 12	1 – 14	• Recombinant proteins

*** Recommended working /cleaning pH. Choose acid /alcoholic solutions for cleaning

**** Short period of time

Click Here To Download:
•Datasheet: Sorbent Selection Guide

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