Comparative Evaluation Of Automated Flash Chromatography And Preparative HPLC For Bench-Scale Purification Of A Broad Range Of Sample Types
The goal of a purification chemist is to deliver suitable quantities of high purity compounds in the shortest possible time. Two of the most popular purification techniques are Automated Flash Column Chromatography (AFCC) and Preparative HPLC. Traditionally, AFCC is characterized by the ability to load large amounts of material and has short purification times, while Prep HPLC is valued for high resolution separations resulting in very pure products. As a result, AFCC is often used as a preliminary technique whereby the crude sample is pre-purified before final high resolution purification using Prep HPLC. By Bopanna N.K., Chitra Sundararajan, and Mark Jacyno
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