White Paper

Achieving Ultra Fast Liquid Chromatography

Source: Shimadzu Scientific Instruments

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•White Paper: Achieving Ultra Fast Liquid Chromatography

Today's demand for increased efficiency in the analytical industry has highlighted the important technical challenge of providing faster separation in HPLC. The direct approach is to increase the mobile phase flow rate (linear velocity); however, increasing the mobile phase flow rate in the widely used 5µm particle diameter packed column diminishes column efficiency. For enhancing performance, there are multiple possible approaches but the following two seem the most promising:

  • Use of smaller particle packing material
  • Separation under elevated temperature
The common goal of the two approaches is to reduce the Height Equivalent to Theoretical Plate (HETP) and, consequently, to increase the column performance per a given column length in a high flow-rate region. There are several factors to consider in both approaches.

Click Here To Download:
•White Paper: Achieving Ultra Fast Liquid Chromatography