Application Note

Automatic Microviscometry As A Tool For Fast Biopolymer Analysis Compared To AF4

Source: Anton Paar USA

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Application Note: Automatic Microviscometry As A Tool For Fast Biopolymer Analysis Compared To AF4

By Anton Paar USA

The aim of this study was to evaluate micro-volume viscosity measurements of high molecular weight bio-polymers for an improved preparation of nanoparticles. Viscosity itself derives from the inner friction of the polymer molecules and is defined as the resistance which a liquid builds up against the urge of flowing. In theory, by extrapolating the viscosity of coiled polymers to an angle alpha=0, the molecular weight of macromolecules can be determined. Molecular weight and distributions have an important influence on the uniformity of the resulting nanoparticles. The results were compared with asymmetrical flow field-flow fractionation (AF4) coupled with multi-angle-light scattering (MALS).

All microviscometry experiments were performed using an AMVn microviscometer (Anton Paar GmbH, Ostfildern, Germany). Gelatine and diethylamino-ethanol-dextran were obtained from Sigma Aldrich (Munich, Germany). Prototype gelatines were donated by Gelita AG (Eberbach, Germany). Nanoparticles from the polymers were prepared according to the two-step desolvation technique described by Coester.

This technique is based on a parabolic flow profile of the sample liquid inside a separation channel with an applied cross-flow determining the retention time of the molecules. AF4 was performed using an AF1000-FOCUS system (Postnova, Landsberg, Germany) coupled with UV- and RI-detection as well as a SLS-detector (Wyatt, Santa Barbara, USA) for molecular weight determination.

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Application Note: Automatic Microviscometry As A Tool For Fast Biopolymer Analysis Compared To AF4