Progressive advances in media optimization and improved clone genetic selection have underscored the perceived performance limitations of single-use bioreactors (SUBs). This study presents how strategic enhancements to the sparge and agitation systems of SUBs have revealed the potential for a three- to four-fold improvement in mixing and mass transfer performance compared to legacy SUB designs.
Adherent cell cultures, such as those with Vero cells, are often more challenging than their suspension-based counterparts with scale-up methods that require optimization of agitation and other operating parameters. This application note presents how single-use bioreactors (SUBs) can be enhanced for scaled-up growth of adherent cells on microcarriers in serum-free medium, in culture volumes of 50 L, 250 L, and 500 L.
With many end users now demanding streamlined operations, high-density culture in fed-batch modes, even higher production by perfusion cultures, and contrastingly gentle operations required in microcarrier applications, standard SUB (single-use bioreactor) offerings must keep pace with the ever-evolving needs of the bioprocessing industry. Read about recent enhancements that are helping keep up with these increasing demands.
As customers create increasingly demanding processes, legacy single-use bioreactors (SUBs) need modification to accommodate higher mixing and mass transfer, supporting higher oxygen demands in aggressive fed-batch cultures. This study presents how strategic enhancements of SUBs have improved mixing and mass transfer performance compared to legacy SUB designs.
This single-use bioreactor was designed to accommodate modern cell culture processes and has features that will help you save time and costs and enable your bioprocessing and scale-up success. Watch this short video introduction to learn more.