Newsletter | September 11, 2020

09.11.20 -- Considerations For Delivering Therapies To Market Faster

Achieving Unique Synergies In Antibody Expression

Shortening timelines to generate more productive, stable, and high-expressing cell lines is the goal of any cell line development program. This technical poster details the methodologies and clonal variations of combining a cell line development technology with a platform for clonal cell line selection.

Optimizing Titer And Use Of A Specific Enzyme For Generating A Fully Processed Antibody

The number of more complex biopharmaceuticals in development continues to increase, and some of these include molecules that require multiple proteins to be expressed at differing ratios to produce the correct biopharmaceutical. In this article, the authors outline cell line development and process scale-up for an antibody program in which the antibody requires additional processing by a site-specific enzyme for correct functionality.

Novel Purification Tool And Its Effects On Host Cell Protein Reduction And Turbidity In Low Isoelectric Point Target Proteins

The 3M Emphaze AEX Hybrid Purifier is a novel purification tool that utilizes a Q-functional hydrogel and 0.2 µm membrane to reduce impurities in a biopharmaceutical downstream process. This study involved a design of experiment (DoE) approach to investigate the effects of pH and conductivity on the turbidity, yield, and impurity clearance of the Emphaze AEX Hybrid Purifier and the efficiency of the subsequent protein capture step.