Application Note

Design Of Experiments For Optimal Production Of Malaria Vaccines

Source: Sartorius Stedim Biotech

The aim of a joint project of the HAW Hamburg and the Biomedical Primate Research Center (BPRC) was to identify optimal protein expression conditions for high yields of potential malaria vaccines in the methylotrophic yeast Pichia pastoris.

Optimal expression conditions vary in accordance to the kind of Pichia pastoris strain used for the foreign protein expressed. As strain characterization and target protein expression optimization need a large number of cultivation runs, a fully automated multibioreactor plant was designed and built up for the implementation of investigations via Design of Experiments (DoE).