ImageXpress Confocal HT.ai High-Content Imaging System

Capture large 3D organoid and spheroid images with up to double the speed

The ImageXpress® Confocal HT.ai High-Content Imaging System utilizes a seven-channel laser light source with eight imaging channels to enable highly multiplexed assays while maintaining high throughput by using shortened exposure times. Water immersion objectives improve image resolution and minimize aberrations so scientists can see deeper into thick samples.

The powerful combination of MetaXpress® software and IN Carta® software simplifies workflows for advanced phenotypic classification and 3D image analysis with machine learning capabilities and an intuitive user interface.

• Enable greater assay flexibility

  Eight imaging channels with laser excitation enable more assay flexibility, higher image brightness, and flexibility to use targeted imaging such as QuickID. Automated Water Immersion objectives offer greater numerical aperture and matched refractive index between the sample and the immersion media for enhanced resolution and decreased aberrations.

• Increase throughput with higher quality images

  Higher excitation power provides increased signal, shorter exposure times, and faster acquisitions of 3D samples. Micro-lens enhanced spinning disk confocal provides a flat field of view for more accurate and reproducible image analysis. Shorter exposure times generates up to a two-fold boost in scan speed. FRET experiments using lasers for CFP and YFP expand research.

• Accelerate analysis speeds

  IN Carta Image Analysis Software performs complex segmentation and classification. Phenoglyphs provides a robust trainable classification, and SINAP provides trainable segmentation for any image type. Accelerate analysis speeds by 40X with multi-threaded, parallel processing with MetaXpress® PowerCore Software. Reduces time from hours to minutes, eliminating 3D analysis as a bottleneck.

Features

• High-intensity laser light source

  Capture images faster with shorter exposure times. Multiplex your experiments with 7 lasers and 8 imaging channels.

• Accurate 3D measurements

  Custom Module Editor 3D analysis is optimized for confocal imaging, enabling 3D measurements of volume and distance.

• Wide field of view

  Wide field of view enables whole-well confocal imaging and eliminates missed targets. Next-generation dual microlens enhanced spinning disk technology provides a large, flat field of view for more accurate and reproducible analysis.

• Exclusive AgileOptix™ spinning disk technology

  Provides increased sensitivity with specially designed optics, high-powered laser illumination, and sCMOS sensor. Swappable disk geometries provide flexibility between speed and resolution.

• IN Carta Image Analysis Software

  Leverages machine learning to improve the accuracy and robustness of high-content image analysis, delivering data insights that other technologies miss. Reduces the complexity of image analysis with intuitive guided workflows in a modern user interface.

• Multiple imaging modes

  The system offers phase contrast and brightfield label-free imaging, widefield and confocal fluorescent imaging with water immersion optics as a standard option.

• Automated water immersion objective technology

  Offers greater image resolution and sensitivity with up to 4x increase in signal leading to lower exposure times.

• Wide dynamic range

  Quantifies low and high intensity signals in a single image with >3 log dynamic range intensity detection.