Quantitative Analysis Of Benzphetamine In Human Plasma Using LC-MS/MS

By William G. Sawyers and Paul S. McCafferty

Benzphetamine hydrochloride is a sympathomimetic amine with pharmacologic activity similar to the amphetamine class of compounds. Presented is data from the bioanalytical validation of a high performance liquid chromatography, tandem mass spectrometry (HPLC-MS/MS) method for the analysis of benzphetamine in human heparanized plasma. Sample preparation was accomplished using a liquid-liquid extraction with chromatographic separation using a C-8 column. The relationship between benzphetamine concentration to peak area ratio (drug/internal standard) was established over the range of 0.050 to 100 ng/mL. Routine use of this method with clinical samples has shown this method to be rugged, sensitive, specific, precise, and accurate.