2D Micro-HPLC System For Proteome Analysis

The high performance 2D micro-HPLC system can perform seperation of complex peptide mixtures with a level of sensitivity and resolution that meets or exceeds present-day requirements.

This high-performance 2D capillary-to-microflow HPLC system co-developed by Shimadzu and the NIH (National Institutes of Health) performs seperation of complex peptide mixtures with a level of sensitivity and resolution that complements present 2D gel electrophoresis.

Features

It is possible to analyze proteins that cannot be analyzed with 2D gel electrophoresis

Highly iconic proteins and hydrophobic proteins can be analyzed on-line.

Automatic analysis with high resolution and sensitivity is possible for proteins with low expression levels

Protein digests can be analyzed automatically by simply placing them into an autosampler. Assay sensitivity enables trace peptide analysis not always detected on 2-D gels.

Stable analysis is ensured by on-line sample trapping and automatic trapping and automatic desalination followed by RPHPLC

A sample from the cation exchange column is trapped on 6 mini trap columns (polymer packed reversed-phase columns with a rentention power equivalent to C4) for desalination, followed by reversed-phase analysis. This method ensures stable, precise analysis.