A Rapid, Scalable Lentiviral Vector Purification Platform With Improved HCP Clearance Using Capto™ Core 700 From Cytiva

As lentiviral vectors continue to advance toward in vivo clinical applications, downstream purification strategies must deliver both exceptional purity and strong infectious titer recovery—at scale. This work explores an integrated downstream processing platform that pairs a high‑selectivity ion‑exchange capture step with multimodal flow‑through polishing to address these demands. By reducing impurity burden early in the process, the platform enables high‑capacity polishing without compromising recovery, even under intentionally stressed, high‑impurity conditions. Detailed breakthrough analysis, comparative capture benchmarking, and quantitative recovery data illustrate how this tandem approach supports consistent host cell protein clearance, robust vector recovery, and scalable performance across the full workflow. For teams developing or refining lentiviral vector manufacturing processes, this platform offers practical insight into how capture and polishing synergy can unlock higher throughput and cleaner product profiles suitable for in vivo delivery.