A Simple And Accurate Method To Quantify Molar Concentrations Of NGS Libraries For Illumina Sequencers

Accurate quantification of NGS libraries is essential for successful sequencing runs, especially on high-capacity platforms like Illumina NovaSeq. NuQuant offers a streamlined, size-independent method for measuring molar concentration using a fluorescent label incorporated during library prep. Unlike qPCR and Bioanalyzer, NuQuant eliminates the need for fragment size analysis and delivers consistent results across varied library types. It correlates strongly with sequencing read counts (R = 0.97) to ensure balanced pooling and minimize the risk of underrepresented samples. With a six-minute workflow and compatibility with standard fluorometers, NuQuant significantly reduces time and cost.

Delve into the full application note to compare NuQuant’s performance to traditional methods and its advantages in accuracy, simplicity, and scalability.