A Simple Method For Cell Growth Media Preparation Using LevMixer™ 10 L Single-Use Mixing System And Supor™ Prime Filters

By Stijn De Backer, Mustapha Hohoud, Valeria Mastrullo, and Sabrina Cencig, Cytiva

Sterility in viral vector (VV) production hinges on aseptic handling, especially during cell growth media preparation—a step that directly influences yield and regulatory compliance. Choosing between liquid and powder media formulations can significantly affect process efficiency. Powdered media offer key advantages: longer shelf life, reduced contamination risk, and flexible reconstitution. These benefits translate into lower storage and production costs. For HEK293 cell lines, both suspension and adherent culture formats are supported by tailored media options that promote high transfection efficiency and viral titers. Despite the critical nature of this step, formal guidelines for risk mitigation during media prep are lacking.

Gain insight into a streamlined approach using levitating mixing and sterile filtration to help ensure sterility and scalability in your VV workflows.