Antibody Analysis Using The Agilent ProteoAnalyzer System

Reliable antibody characterization depends on analytical methods that can address complex molecules without slowing laboratory workflows. Automated parallel CE-SDS enables high-resolution assessment of protein sizing, purity, fragmentation, and size heterogeneity under reduced and nonreduced conditions. Practical examples demonstrate the use of NISTmAb as a performance benchmark and sizing ladder, the complementary value of SEC and CE-SDS, and the characterization of cysteine- and lysine-conjugated ADCs. Additional comparisons examine fluorescence and UV detection, with guidance for optimizing buffers, injection conditions, incubation temperatures, and sample handling. The Agilent ProteoAnalyzer system processes up to 12 samples in parallel, helping laboratories produce reproducible digital data with less hands-on work.

Access the full application compendium to strengthen antibody research and quality control workflows.