Automated, Rapid, Reproducible Quantification Of HIV-1 Gag p24

Introduce Simple Plex into Your Cell & Gene Therapy Workflow

With the growing use of Lentiviral vectors derived from HIV-1 in cell and gene therapies, the need for precise calculations of viral titres during throughout the manufacturing process is crucial.

Reliable methods for quantification of Lentiviral vectors support process development and enables quality control of both vector and cell manufacturing. Traditionally ELISA assays serve as a common methodology for calculating viral titres and are used to measure the amount of viral capsid p24 protein in the supernatant of packaging cell lines.

The 3.5 to 6 hours required to run a Standard ELISA is a challenge for operators who who increasingly require results quickly to inform real time decision making.

The Simple Plex p24 assay, hosted on Ella - an automated immunoassay system, can rapidly quantify p24 levels expressed from lentiviral vectors during different stages of cell and vector manufacturing. The automated, robust and reproducible nature of the Simple Plex p24 assay removes user error, and makes it amenable for GMP environments when quality, standardisation of process, assay transfer and scalability are highly desirable.

Simple Plex Assays are also designed to fit multiple assay requirements. Use our custom assay development service or choose from >180 available off the shelf biomarkers to create your single or multiplex assay formats.