Cell Culture Media Demystified For Performance, Quality, And Innovation

By Laura Prescott, Marketing Writer, Cytiva

Cell culture media can make or break your bioproduction process, but understanding what’s inside that “black box” is key to unlocking better performance. This article explores how media composition affects cell growth, productivity, and product quality, with insights from four cell culture experts. It breaks down the types of media available, from classical to chemically defined and custom formulations, and explains how each impacts reproducibility and scalability. You’ll learn how boosters and enhancers can help, but why custom media tailored to your cell line may be the most effective path forward. Gain insight into analytical approaches like spent media analysis and design of experiments (DoE) that can guide optimization. Whether you're working with HEK293 or CHO cells, understanding your media’s role is essential.

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