Article | June 11, 2019

Chromatography Scale-up: Don't Get Tied Down By Bed Height

Source: Cytiva

By Kerstin Erickson , Senior Research Engineer at GE Healthcare Life Sciences

Bioprocessing

Goodbye bed height, hello bed volume

Keeping a constant residence time is a well-established strategy when scaling up a chromatography step. Typically, this is achieved by keeping the bed height and linear flow velocity constant. But aiming for a constant bed height throughout scales has a limitation—you need an exact chromatography column size that fits the purpose.

If the column is too small, you waste time by cycling it several times to reach the desired output. If the column is too big, you don’t use all the capacity you have and waste resin. So, I offer you an industry hack: standardize across scales to constant column volumes per hour (CV/h) instead.

VIEW THE ARTICLE!
Signing up provides unlimited access to:
Signing up provides unlimited access to:
  • Trend and Leadership Articles
  • Case Studies
  • Extensive Product Database
  • Premium Content
HELLO. PLEASE LOG IN. X

Not yet a member of Pharmaceutical Online? Register today.

ACCOUNT SIGN UP X
Please fill in your account details
Login Information
I'm interested in newsletter subscriptions.
ACCOUNT SIGN UP

Subscriptions

Sign up for the newsletter that brings you the industry's latest news, technologies, trends and products.

You might also want to: