Cold Storage And Cryopreservation Animal Component Free Solutions For Short And Long Term Storage Of Cells

By Mira Genser Nir, Meital Gury Ben Ari, Anat Vishlitsky, Nyra Goldstein, Sharon Daniliuc, Marina Teverovsky, Yuliya Yael Miropolski, Roni Hazan Brill, Natali Malka, Maria Sharovetsky, Bar Izkovich, and David Fiorentini

Discover two innovative solutions for cell storage: NutriFreez® D5 and NutriStor®. NutriFreez® D5 is a cryopreservation solution with reduced DMSO concentration (5%) designed for long-term storage at ultra-low temperatures. It ensures high viability and recovery of human mesenchymal stem cells (hMSC) and embryonic stem cells (hESC), maintaining essential cell properties post-thaw. Compared to other cryopreservation solutions, NutriFreez® D5 offers similar or superior performance while minimizing the potential cytotoxic effects of DMSO.

NutriStor® is a cold storage solution that supports short-term cell storage at 2-8°C without freezing, ideal for therapeutic applications and shipment. It eliminates the need for toxic cryoprotectants, reducing risks associated with cell necrosis and apoptosis. NutriStor® ensures stable storage and high recovery rates for various hMSC sources, with comparable results to cryopreserved cells. This flexibility makes it suitable for both suspension and monolayer cultures, meeting the demands of diverse applications.

This poster emphasizes the importance of optimizing storage solutions to maintain cell integrity and functionality, ensuring reliable outcomes in cellular therapies. The use of chemically defined, animal-component-free solutions in both NutriFreez® D5 and NutriStor® ensures high-quality storage while meeting regulatory requirements.

Future developments aim to introduce DMSO-free cryopreservation solutions with non-toxic cryoprotectants, further advancing the safety and efficacy of cell-based products. These innovative storage solutions demonstrate the potential to improve scalability and logistics in regenerative medicine and other cell therapy applications.