News | October 12, 2001

GeneMachines new RevPrep Orbit workstation achieves successful nucleic acid purification results during beta program

Lawrence Berkeley National Laboratory (Berkeley Lab) plays a key role in establishing the newly redesigned RevPrep Orbit workstation as a viable solution for low-cost sample preparation

GeneMachines®, a leading genomics and proteomics automation provider, today announced that the new RevPrep Orbit™ workstation achieved successful results in beta tests of its nucleic acid purification capabilities. The RevPrep Orbit automates genomic sample preparation, a process that, when undertaken manually, is repetitive, labor intensive and costly. By using the new RevPrep Orbit, laboratories can both increase throughput and decrease cost.

GeneMachines beta partner, the Berkeley Laboratory's Drosophila Genome Center, incorporated RevPrep Orbit into its around-the-clock DNA purification schedule. No protocol optimization efforts were made to accommodate the RevPrep Orbit in the Berkeley Lab production line. During unattended production, Berkeley Lab purified 24 96-well plates of plasmids with set-up occurring once in the morning and once again before leaving for the day. This throughput is easily handled by the RevPrep Orbit. When judged against the competition, the RevPrep Orbit had fewer lane failures, fewer failed plates, and comparable read lengths. Reed George, Manager of the Technology Development Group at the Berkeley Laboratory praised both performance and cost savings, "The RevPrep Orbit system has consistently yielded high quality DNA in our sequencing operations and has had a major impact in our cost reduction efforts."

The RevPrep Orbit workstation features the unique Array Centrifuge™ technology, exclusively licensed from Stanford University (SUTECH®, a trademark of Stanford Genome Technology Center). The Array Centrifuge contains 96 individual rotors, within which occur all necessary sample-processing steps. A validated alkaline lysis prep protocol provides purified DNA of optimized yield and purity for compatibility with capillary electrophoresis sequencers. The foremost advantage of this technology over conventional filter-based preparation methods is the significant cost savings; no disposables such as filter plates are required.

The RevPrep Orbit workstation is the newest addition to the GeneSuite® product line of automated solutions, serving the needs of genomics and proteomics researchers.

About GeneMachines
Incorporated in 1997, GeneMachines (www.genemachines.com) provides state-of-the-art solutions for the automation and instrumentation needs of the genomics and proteomics communities. Based in San Carlos, CA, the company is headed by Scott Hunicke-Smith, Ph.D., founder and CEO. In response to rapid growth in the fields of genomics and proteomics, GeneMachines has developed the GeneSuite, a product line of integrated instruments, reagents, and consumables meeting the high-throughput instrumentation needs for both sample preparation and functional analysis.

GeneMachines' mission is to accelerate the advancement of knowledge in the life science arena. GeneMachines collaborates with researchers to determine and address instrumentation needs and challenges. To inquire about the company's growing line of products and services, please call 877-855-GENE (4363) (US) or 1-650-508-1634 (International), or e-mail info@genemachines.com.

About Berkeley Laboratory
Berkeley Laboratory is a U.S. Department of Energy national laboratory located in Berkeley, California. It conducts unclassified scientific research and is managed by the University of California.

Source: GeneMachines