High-Throughput Pyrogen Testing In A Multimode Microplate Reader

Pyrogen detection is essential for ensuring the safety of pharmaceutical products, yet traditional methods like the Rabbit Pyrogen Test or cytokine‑based MAT workflows can be slow, variable, and resource‑intensive. Explore a streamlined alternative that pairs an NF‑κB–responsive luciferase reporter assay with a multimode microplate reader to deliver rapid, highly sensitive pyrogen testing. The workflow reduces assay time from roughly two days to about five hours while maintaining robust detection of both endotoxin and non‑endotoxin pyrogens. Validation studies highlight strong accuracy, minimal interference, and reproducibility across challenging sample types, including those known to complicate LAL‑based testing.

For teams seeking faster turnaround, higher throughput, and more confidence in pyrogen screening, access the full application note for detailed methodology, performance data, and validation guidance.