Impact Of A Chemically Defined Medium For Vero Cells Cultivation And Virus Production For Vaccine Applications

By Roni Hazan Brill, David Fiorentini, Ber van der Wijden, Bella Monica, Gerco van Eikenhorst, Emilie Rodrigues, Yvonne E. Thomassen, Adrien Lugari, Maverick Lau, and Érica A. Schulze

In the race to create serum-free, chemically defined media, research efforts have focused on identifying the elements required for superior growth performance. This include growth factors, hormones, carrier proteins, lipids, transition metals, vitamins, polyamines, and adhesion factors. This application note describes 4Cell® NutriVero™ Flex 10, a new Vero cell medium that is serum-free, chemically defined, and animal component-free. 4Cell® NutriVero™ Flex 10 delivers performance that matches conventional media with undefined hydrolysate supplementation.

4Cell® NutriVero™ Flex 10 has been developed to support Vero cell growth in monolayers and microcarrier suspension culture systems and is optimized for the production of viruses such as measles, Sabin poliovirus Type 1, and enterovirus 71. The Vero cell line is one of a very limited number that have been approved by health authorities worldwide for the production of human vaccines. Its record of safety, quality, and quantity of viral yield is well documented.