In Vitro/In Vivo Correlation Of Permeability Of Standard Compounds Using 4 Different Cell Lines

By Narayanasamy Duraisamy, Mahadevaswamy G. Kuruburu, Sourav Maity, Chinmayee Sahoo, Avinash M Govindaraju, Nikita Karpe, Bhupathi Jillela, and Pratima Srivastava

Cell monolayers are a prevalent tool in drug permeability screening during drug discovery, as they simulate the physical and biochemical characteristics of biological barriers relevant to drug absorption.

This study employed a comparative approach using several cell lines – Caco-2 (human colorectal cancer), LLC-PK1 (porcine kidney), MDCK-II (Madin Darby canine kidney), and MDR1-MDCK-II (MDCK-II expressing MDR1 protein) – to investigate permeability and transporter activity within each line. The assessment utilized established standard compounds: Propranolol (high permeability), Atenolol (low permeability), and Digoxin (P-gp substrate).