Integrated LC/UV And LC/MS Workflows For Impurity And Degradation Characterization Of GLP-1 Agonists

Fatty acid moieties extend the half-life of GLP-1 receptor agonists, but they also make these 30 to 40 residue peptides harder to separate and characterize. David L. Wong and Suresh Babu C.V. of Agilent Technologies pair LC/UV with single quadrupole and Q-TOF mass spectrometry to examine tirzepatide and retatrutide, including thermally stressed samples. The work reports reproducibility data for the UV method, deconvoluted masses that reveal minor impurities and isomeric variants, and a comparison of semaglutide, liraglutide, tirzepatide, and retatrutide purity profiles. Samples from multiple suppliers show how impurity patterns can differ, a point with direct bearing on supplier qualification.

Delve into the chromatograms and spectra to see how the workflow performs on these molecules.