Intensified And Improved Fed-Batch Production Process: High-Density N-1 Perfusion

Data presented in this article is a proof of concept to demonstrate how using ReadyToProcess WAVE™ 25 bioreactor and an intensified N-1 perfusion seed culture approach can support a high-density seed in a production bioreactor. A high-density perfusion replaced the conventional N-1 seed train step, and compared to conventional runs, the production bioreactor can be seeded at a 10-fold higher seed density.

The process allows for the intensification of your fed-batch process through reduced production duration, and there is no effect on the growth or titer profile. In addition, the number of runs per year that can be scheduled in a production facility can be increased without the expansion to the production bioreactors quantity.

Chinese hamster ovary (CHO) cells expressing a monoclonal antibody were grown in HyClone™ brand ActiPro medium and fed with HyClone™ brand Cell Boost Supplement 7a and Cell Boost™ Supplement 7b. Batch cell culture was initially performed in shake flasks (SF) and then scaled to ReadyToProcess WAVE™ 25 bioreactor cultures in either batch or perfusion before transfer to Xcellerex™ XDR-500, a production bioreactor.