LC-MS/MS Method To Analyze 20 Amino Acids

Amino acids are the building blocks of proteins and peptides in biological systems. Amino acids have also been used as supplements to support health and as indicators for certain diseases. The wide ranging polarities spanning across all 20 amino acids makes the analysis challenging.

Due to this complexity, methods in the past have relied on derivatization of the amino acids; however, derivatization can lead to further complexity because of the presence of derivatized/underivatized amino acids and interferences from the derivatizing reagent itself. Read about a LC-MS/MS method for analyzing all 20 amino acids, without derivatization, utilizing the Supel™ Carbon LC column.