Current destructive methods for moisture determination of lyo product vials, such as Karl Fischer titration, are not fit for statistical studies. Rapid non-destructive headspace moisture analysis from LIGHTHOUSE enables fast moisture determination of statistical numbers of lyo samples. Due to the non-destructive nature Lyo Chamber Moisture Mapping, valuable product samples remain intact and can be used for other purposes. Process development and scale-up groups wanting deep insight into lyo cycle efficiency, batch homogeneity, and freeze dryer performance can benefit greatly from headspace moisture analysis platforms.