Explore the mRNA universe with Cytiva. In this series, Cytiva scientists share their journey and insights on mRNA synthesis for research purposes and they share their advice on how to scale.
The creation of an encapsulated mRNA–LNP begins with synthesizing the DNA template. If plasmid DNA (pDNA) is used as the template, additional steps are necessary, such as purification after host cell lysis and linearization of the circular pDNA. The DNA template then undergoes purification prior to in vitro transcription (IVT), during which mRNA is formed.
Explore the DNA template, DNA template synthesis, the challenges and strategies of generating a DNA template using PCR, and more.