Fetal bovine serum (FBS) is used as a media supplement for most adherent in vitro cell culture applications in academia and industry. FBS from a single origin may be limited, so qualifying FBS from other origins is one way to enhance security of supply.
We evaluated and compared HyClone characterized FBS originating from the United States, New Zealand, and Australia for cell culture and production performance. Experiments used adherent HEK293T cells with HyClone DMEM supplemented with 10% FBS and examined:
- General morphology
- Cell growth kinetics and population doubling times
- Transfection efficiency after PEI-based adeno-associated virus serotype 2 (AAV2) transfections
- Virus production after PEI-based AAV2 transfection