Real-Time Growth Monitoring Of Spheroids In Round Bottom Microplates

Three-dimensional cell culture models are transforming how researchers study drug efficacy, disease progression, and tissue engineering. Explore a fully integrated workflow for generating and monitoring spheroids in ultra-low attachment round-bottom microplates. Using label-free brightfield and fluorescence imaging, the Spark Cyto multimode reader enables real-time kinetic analysis of spheroid growth, supported by automated staining and fixation via the HydroSpeed plate washer. The system’s image-based autofocus and AI-driven segmentation allow precise tracking of morphological changes and fluorescence intensity over time, even across varying spheroid sizes and densities. Data visualization tools provide immediate feedback, helping researchers identify optimal timepoints for intervention. The workflow is designed to reduce manual handling, improve reproducibility, and streamline 3D assay development.

Explore the full application note to see how automation and imaging precision can elevate your 3D cell culture workflows.