Simplify Cell Line Development

Efficient monoclonal cell line development is essential for applications ranging from therapeutic protein production to gene therapy research. This application note presents a streamlined workflow that combines the Uno Single Cell Dispenser and Spark Cyto multimode imaging reader to simplify and accelerate single cell isolation and viability assessment. The Uno system uses microfluidic digital dispensing to gently isolate individual cells, achieving a 97% success rate across 80 wells. Spark Cyto complements this by enabling whole-well imaging and confluence analysis, confirming 90% cell viability and 47% outgrowth after 10 days. Together, these tools offer a reliable, scalable alternative to traditional methods like limiting dilution and FACS, reducing complexity while preserving cell health. Researchers can adapt this approach across a range of cell-based applications to improve reproducibility and streamline development timelines.

See how this integrated solution supports high-confidence monoclonality and robust clone characterization.