Simplify Cell Line Development: Combining Uno Single Cell Dispenser™ And Spark® Cyto Multimode Imaging Reader To Accelerate Cell Line Development

Single-cell cloning plays a pivotal role in developing monoclonal cell lines for applications ranging from therapeutic protein production to drug screening and gene therapy. Achieving true clonality—ensuring each colony originates from a single cell—is essential for consistent product quality and reproducible results. Traditional methods like limiting dilution and FACS come with limitations, including inefficiency, high cost, and potential impact on cell health. This application note introduces a streamlined approach using the Uno Single Cell Dispenser and Spark Cyto imaging system. Together, they enable gentle, precise isolation of viable single cells and straightforward assessment of outgrowth.

Discover how this combination offers a simplified workflow for researchers seeking reliable monoclonal cell line development.