Product/Service

TaKaRa Chaperone Plasmid Set

Source: Takara Mirus Bio
The Chaperone Plasmid Set consists of 5 different plasmids, each of which is designed to express multiple molecular charperones that function as a "chaperone team" to enable protein folding. It has been reported that coexpression of a target protein with one of these chaperone teams increases recovery of proteins in the soluble fraction...
FEATURES
  • Each plasmid is designed for expression of multiple molecular chaperones known to aid protein folding
  • Recovery of proteins in the soluble fraction is increased
  • Plasmids carry an origin of replication derived from pACYC and a chloramphenicol resistance gene (Cmr): can be used with E. coli expression systems utilizing ColE1-type plasmids with the ampicillin resistance gene as a marker
  • Chaperone genes are located downstream of araB or Pzt-1 (tet) promoters: expression of target proteins and chaperones can be induced individually if the target gene is placed under the control of other promoters (e.g. lac)

DESCRIPTION

The Chaperone Plasmid Set consists of 5 different plasmids, each of which is designed to express multiple molecular charperones that function as a "chaperone team" to enable protein folding. It has been reported that coexpression of a target protein with one of these chaperone teams increases recovery of proteins in the soluble fraction. The plasmids carry an origin of replication derived from pACYC and a Cmr gene, which allows use with E. coli expression systems utilizing ColE1-type plasmids with an ampicillin resistance gene as a marker. The chaperone genes are situated downstream of an araB or Pzt-1 (tet) promoter, thus expression of target proteins and chaperones can be induced individually if the target gene is placed under the control of other promoters (e.g. lac). These plasmids also contain the necessary components (araC or tetR) for each promoter.

Note that this system cannot be used in combination with chloramphenicol-resistant E. coli host strains or expression plasmids that carry a chloramphenicol-resistant gene. For example, E. coli BL21(DE3), which is often used with pET systems, is acceptable as a host strain, however E. coli BL21(DE3) pLysS and BL21(DE3) pLysE, which contains pLysS or pLysE plasmids that have the pACYC replication origin and the Cmr gene, cannot be used with this system.

Other applicable hosts include E. coli JM109.

Click here to download the Plasmid Maps and Components.