The Bright Side Of Biologic Stability: A Thermofluor Screen For Rational Buffer Optimisation

By Jacob Poxon and Jordan Potts, Upperton Pharma Solutions

Therapeutic proteins, including monoclonal antibodies (mAbs), are increasingly central to modern medicine, yet their structural complexity makes them highly susceptible to denaturation, aggregation, and chemical degradation — issues that can compromise both safety and efficacy. Stabilizing these biologics through carefully designed injectable buffers is critical, but the process is often complex and resource-intensive. Traditional screening methods, such as buffer exchange and circular dichroism, are low-throughput, time-consuming, and costly.

At Upperton Pharma Solutions, we highlight the promise of thermal shift fluorimetry (thermofluor) as a rapid, low-volume, and cost-effective technique to accelerate buffer optimization and enhance protein stability. Explore how thermofluor screenings can streamline formulation development and unlock greater biologic stability.