The Titer Gap – A Holistic Approach To Understanding High And Low HEK293 Producer Cell Lines In AAV Production Processes

By Niklas Krämer , Vera Ortseifen, Luisa Vetter, Kathrin Teschner, Alyssa Vetter, Tim Steffens, Stefan Northoff, and Sandra Klausing

Despite significant advancements in Adeno-Associated Virus (AAV) -based gene therapy, several critical aspects of AAV biology and production remain poorly understood. Researchers are actively working to identify host cell characteristics that enhance product yield in viral vector production processes. Given the diversity of HEK293 subclones, each with unique genetic modifications, comparing a cell line known for producing high viral titers with one that generates fewer viral vectors can provide valuable insights into the cellular factors influencing various stages of AAV production. The ongoing research aims to unravel the complexities of AAV biology to focus on optimizing production efficiency and understanding the underlying mechanisms. By examining different HEK293 subclones, scientists hope to pinpoint specific genetic or cellular traits that contribute to higher viral vector yields.

Find out how understanding the interplay between host cell characteristics and AAV production can pave the way for developing tailored cell lines that maximize viral vector output.