Transfer Of Fed-Batch To Semi-Perfusion Process Using Automated Small Scale Bioreactors

By Sabrina Janoschek, Markus Schulze, Gerben Zijlstra, Gerhard Greller, and Jens Matuszczyk, Sartorius

Continuous processes offer several advantages over traditional batch approaches, such as enhanced product quality, increased yield, and significant cost savings. This application note details the utilization of the Ambr® 15 automated, high-throughput microscale bioreactor system, which features perfusion mimic capabilities, to transition a fed-batch process to perfusion using a semi-perfusion protocol. Various cell culture conditions were meticulously examined and optimized, including medium and feed composition, glucose concentration, and the cell-specific perfusion rate. Following optimization, the protocol was successfully transferred to the Ambr® 15 system, which allowed for a comprehensive comparison of process performances.

By employing the same platform medium and proprietary feeds, a robust yet resource-efficient method was developed to convert an existing CHO fed-batch process to a perfusion process. Explore more about the suitability of this process for the Ambr® 15 system, with its perfusion mimic capabilities, as a scale-down model has been convincingly demonstrated.