Walk-Away Monitoring Of Cytotoxicity, Viability, And Apoptosis

Understanding the timing and mechanism of cell death is critical for accurate drug profiling. This application note presents a walk-away workflow for real-time monitoring of cytotoxicity, viability, and apoptosis using Promega’s cell health assays and the Spark® multimode reader. By integrating kinetic assays with automated reagent injection triggered by cytotoxicity thresholds, researchers can pinpoint apoptosis induction windows and distinguish between primary necrosis, secondary necrosis, and apoptosis. The study evaluates six kinase inhibitors on K562 cells, revealing how dose and exposure time influence cell health outcomes. The Spark® platform’s automation and environmental controls enable extended, unattended data acquisition, improving reproducibility and throughput.

For researchers seeking to refine mechanistic toxicity assessments, discover more about this practical and scalable solution.