A New Approach For High-Resolution Full And Empty AAV Capsid Analysis

By Jane Luo, Tingting Li, Sahana Mollah, and Elliott Jones SCIEX, USA

Utilize a high-throughput method for comprehensive AAV evaluation on a single CE platform for high-resolution full and empty AAV capsid analysis.

For drug manufacturers, it is essential to characterize and monitor multiple CQAs to ensure the safety and efficacy of AAV drug products. Different analytical platforms are currently being used to characterize multiple CQAs, and these methods all have limitations or cannot be used to analyze all serotypes without significant optimization. Here your can review an approach for high-throughput multiple CQA analysis of AAVs using a single platform: the multi-capillary BioPhase 8800 system. High-accuracy analytical data was collected in a single day for multiple CQAs, including nucleic acid loading and titer, capsid protein profile and ratio, and capsid titer from the same samples.