Accelerating Gene Edited Cell Lines With Efficient Screening Through Fluorescence Detection

By Prathyushakrishna Macha, PhD | Research Scientist | Molecular Devices

This study establishes an effective and rapid process for developing a p53 knockout HEK-293 cell pool using CRISPR-based gene editing and antibiotic screening. The CloneSelect Imager FL system is meeting the challenges of gene-editing, by effectively imaging cells grown (suspension or adherent) and transfected using fluorescent channels. These images are analyzed to select the highest transfection efficiency and protein of interest expression levels.

The CloneSelect Imager FL system has two fluorescent channels, plus white light for total confluency detection. Multichannel fluorescent imaging significantly improves efficiency and streamlines the workflow, generating a cell pool and eventually a monoclonal cell line with day zero monoclonality tracking.